Biology, 22.08.2020 03:01 raizagisselle1273
You believe that the cytosolic protein p007 is involved in mediating growth hormone-stimulated proliferation in cells. You wish to establish a cell model using your cultured fibroblasts. Provide clear explanations to the following, each worth 2.5 pts each. a) Describe how you would transfect the growth hormone receptor DNA into your cells to create stable cell lines, and include a description of the benefits of using stable cell lines versus transiently transfected cell line. b) Describe how you would measure specific receptor binding of GH to your stable cell line. c) Describe how you would measure growth hormone-induced proliferation in your stable cell line. Make sure to include controls. d) Describe how you would use RNAi to demonstrate the requirement for p007 in growth-hormone stimulated proliferation. Make sure to include controls.
Answers: 1
Biology, 21.06.2019 14:00, Hailey1313131313
How do environmental factors influence genetic traits? a. environmental factors determine all genetic traits. b. environmental factors can determine if a genetic trait becomes a health issue. c. environmental factors are determined by genetic traits. d. environmental factors have no influence on genetic traits. select the best answer from the choices provided
Answers: 3
Biology, 22.06.2019 02:00, winterblanco
The fish shown above is a tarpon. it is a fast-swimming and powerful open-water fish. its closest relatives, oddly, are burrow-dwelling conger eels that stay on the bottom. both eels and tarpon developed from snake-like larvae that float in the plankton during the first stages of life. once they mature, tarpon and eels are not found near one another in the ocean. the tarpon and the eel illustrate all of the following except
Answers: 1
You believe that the cytosolic protein p007 is involved in mediating growth hormone-stimulated proli...
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