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Access link #1 and read the section about light intensity (i0 and i), transmittance (t) and absorbance (a). as described in question 1, an absorbance spectrum is a graph that illustrates the correlation between wavelength and absorbance. the wavelength of maximum absorbance, λmax, is where the absorbance is the greatest in the absorbance spectrum. access the simulation of link #2 to (1) create an absorbance spectrum between 200 nm and 700 nm with 10 to 15 data points, and (2) determine the wavelength of maximum absorbance to within 5 nm. the simulation will you understand the use and function of a uv-vis spectrophotometer in our lab. approach (link #2): run simulation to obtain the transmitted light intensity at various wavelengths (between 200 nm and 700 nm, 10 to 15 data points). each simulation should be run long enough to detect at least 10000 photons. for each wavelength, calculate the transmittance of the solution (light intensity of blank is 3300 photons/second; t = i÷ i0). calculate the absorbance of the solution using transmittance at each wavelength (a = −log10( create the absorbance spectrum by plotting absorbance (y-axis) versus wavelength (x-axis). determine λmax by locating the wavelength at the maximum absorbance.
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Access link #1 and read the section about light intensity (i0 and i), transmittance (t) and absorban...
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