What mistake did Pasteur's assistant make? He didn't refrigerate his cultures.
He waited longer than usual to check for results.
He injected too much of the culture.
He used an old culture of germs.

He used an old culture of germs.

Explanation:

The chickens did not become ill. Pasteur reasoned the factor that made the bacteria less deadly was exposure to oxygen.

He waited longer than usual to check for results.

Explanation:

In 1879, Pasteur observed, by chance, that old bacterial cultures lost their virulence. He had instructed an assistant to inject the chickens with a fresh culture of the viral bacteria before a holiday. The assistant, however, forgot and went on vacation.

Certified answer

the federal constitution of the united mexican states of 1824 was enacted on october 4 of 1824, after the overthrow of the mexican empire of agustin de iturbide. in the new constitution, the republic took the name of of united mexican states. it was replaced by the federal constitution of the united mexican states in 1857.