What land feature stood as a barrier between the thirteen colonies and the ohio river valley

How did the enlightenment change attitudes about existing political systems

Ineed with what i should write for my research paper. my topic is history of slavery. 1800 words. all i need is what to write in each paragraphs. like when the research paper has titles for each paragraph and it explains the tile in each paragraph. for example when did slaverh begin? why did it begin like an outline.

What happened as agriculture became entrenched in the south ?