Which question best reflects the biggest concern that tucker must address for
group c?

The question that best reflects the biggest concern that Tucker must address for Group C is "How can you ensure that animals are treated humanely?"

Explanation:

The question is not complete is missing this part:

"Tucker is giving a speech proposing that more federal money be spent on research labs that conduct animal testing. "

Based on all the information given we can see that every question represents one of the groups and their point of view, for example, Group A worries about the monetary affectations, as Group B worries about the benefit for labs with the budget, like Group D does not have a clear posture towards the subject, then Group C worries about the animal rights, they may make the question "How can you ensure that animals are treated humanely?"

i would pick a. i think this is more of a personal question though.

yeah it is i have the dark knight