Engineering, 19.12.2020 02:40 jaylan11brown
A single stage continuous culture of Corynebacterium glutamicum bacteria is conducted in a 200
L stirred fermenter, at 30oC. The reactor is sparged with a gas mixture of 63% oxygen and 37%
nitrogen at a rate of 1 VVM (Volume/Volume. min) at room temperature. Average pressure in the
fermenter is 1 atm. Volumetric flow rate of off-gas from the fermenter is 189 L min–1. Exit gas
stream from the fermenter has been analysed and found that it contains 52% O2.
a) What is the oxygen transfer rate?
b) If the maximum specific oxygen uptake rate of the microorganism is 5.10-3 g L-1 s-1, is the
cell growth in the fermenter limited by mass transfer? Explain shortly.
Answers: 3
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