8. (4pts) Based upon your estimated purity of the E3 fraction in the question above, and upon knowing the amount of protein present in your fraction, estimate the total yield (in ug) of rGFP obtained using this protocol. You must show/explain how you calculated your answer.

Aplate clutch has a single pair of mating friction surfaces 250-mm od by 175-mm id. the mean value of the coefficient of friction is 0.30, and the actuating force is 4 kn. a) find the maximum pressure and the torque capacity using the uniform-wear model. b) find the maximum pressure and the torque capacity using the uniform-pressure model.

Steam enters a converging nozzle at 3.0 mpa and 500°c with a at 1.8 mpa. for a nozzle exit area of 32 cm2, determine the exit velocity, mass flow rate, and exit mach number if the nozzle: negligible velocity, and it exits (a) is isentropic (b) has an efficiency of 94 percent

Acertain flow of air (at stp) has a velocity distribution given by v i (in ft/s). if this flow is going through a 4 ft square area in the yz-plane (centered at the origin), what is the mass flow rate (in lbm/s)?