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Engineering, 04.03.2020 18:38 martinezarielys17

G Double‑reciprocal, or Lineweaver–Burk, plots can reveal the type of enzyme inhibition exhibited by an inhibitor. Competitive inhibitors increase the K M without affecting the V max . Noncompetitive inhibitors decrease the V max without affecting the K M . Uncompetitive inhibitors decrease both the K M and V max . A Lineweaver Burk plot shows two lines representing the kinetics of an enzyme with and without inhibitor. The x-axis plots the inverse of the substrate concentration, and the y-axis plots the inverse of the reaction velocity. The x-intercept of the line with inhibitor is more negative than the line with no inhibitor. Both lines have the same y-intercept. Based on the Lineweaver–Burk plot, what type of enzyme inhibition is exhibited by the inhibitor?

competitive

noncompetitive

uncompetitive

cannot be determined

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