The Michaelis-Menten equation models the hyperbolic relationship between [S] and the initial reaction rate (vo) for an enzyme catalyzed, single substrate reaction: E + S ES → E + P. The model can be more readily understood when comparing three conditions: [S]<Km. Match each statement with the condition that it describes. Note: "Rate" refers to initial velocity (Vo) where steady state conditions are assumed; [Etotal refers to the total enzyme concentration, and [Efree] refers to the concentration of free enzyme. [S] = Km Not true for any of these conditions almost all active sites are empty Eswl is about equal to Egoud ES] is much higher than [Ereel half of the active sites increasing Eotal will lower Km for most in vivo enzymes this condition rarely occurs are filled with S 0 Incorrect. At leasto approaches a maximum because the rate depends on [ESI, and most of the enzyme is in the ES form.