Engineering, 18.11.2019 23:31 Manglethemango9450

How efficient would pcr be if we set the annealing temperature higher or lower than the calculated melting temperature? how would a higher or lower temperature affect the annealing capability of the primers and the final quantity or quality of the products? how would a gel of such pcr products look compared to pcr products obtained at the optimum annealing temperature?

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How efficient would pcr be if we set the annealing temperature higher or lower than the calculated m...