Q1. Which two chemicals in the table above are most likely act together as a buffer? Support your claim using what you know about buffers and how they work. Q2. What is the difference between the old media and the new media? What impact does this have on the ability of the new media to buffer changes in pH?

Q3. As bacteria grow, they produce acidic metabolic waste products that gradually slow down the rate of cell division. Based on this, which media would you expect to allow the bacteria to grow for a longer period of time before the pH goes up rapidly and cell growth slows down? Include the reasons supporting your prediction.