Defined medium -22 mM glutamate(C5H9NO4)
-20mM NH4Cl
-0.5 mM MgSO4 · 6 H2O
-0.8 mM CaCl2 · 2 H2O
-Trace elements and vitamin solution (sufficient amounts of Fe3+, Mn2+, Co2+, Cu2+, Zn2+, Ni2+, biotin, etc.)
-14 mM potassium phosphate buffer, pH 6.6 to 6.7
how many moles of nitrogen are provided per liter of the medium?