Given a 0.300 M solution of anserine at its isoelectric point and ready access to 0.100 M HCl, 0.100 M NaOH and distilled water, describe the preparation of 1 L of 0.0300 M anserine buffered solution, pH 7.20.
Volume of stock solution needed =
mL
Volume of HCl needed to adjust the imidazole group =
mL
Volume of HCl needed to titrate the amino group =
mL
Total HCl needed =
mL
Pi8.27
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