For an enzyme kinetics experiment, a student prepared a reaction mixture by mixing 450 microliters of 0.75mM PNPP with 4.25ml of 0.2M Tris-HCl buffer. When he is ready to measure the absorbance, he added 0.3ml of Alkaline Phosphatase to the mixture and mixed thoroughly. What is the substrate concentration at the beginning of the reaction in mM
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Chemistry, 22.06.2019 08:30, vanessadaniellet21
Since the gas in your graduated cylinder is a mixture of butane and water vapor, you must determine the partial pressure of the butane, pbutane, alone. to do this, consult a reference and record the partial pressure of the water vapor, pwater, at the temperature you recorded. use the following formula to compute the partial pressure of the butane. pbutane = atmosphere - pwater use the following combined gas law formula and compute the volume that the butane sample will occupy at stp. (hint: convert both temperatures to kelvin.) pbutane x voriginal = pstandard x vfinal troom tstandard use the following ratio and proportion formula to determine the mass of butane needed to occupy a volume of 22.4 l at stp. grams of butane you used “x” grams of butane ml of butane corrected to stp = 22,400 ml compute the theoretical molar mass of butane based on its formula and the atomic masses on the periodic table. compare your experimental results from #3 to the theoretical value of #4, computing a percent error of your findings using this formula: % error = measured value - accepted value x 100 accepted value use the following ratio and proportion formula to determine the mass of butane needed to occupy a volume of 22.4 l at stp. need asap
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For an enzyme kinetics experiment, a student prepared a reaction mixture by mixing 450 microliters o...
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