The gel is loaded as outlined in the LEAPS Laboratory Manual: Lane 1 – Protein Standards; Lane 2 – Induced cell lysate; Lane 3 – Column flow-through; Lane 4 – resin-bound protein; Lane 5 – purified GFP; Lane 6 – Non-induced cell lysate. The group that created this gel were disappointed to see no band in Lane 5. To try and determine what went wrong the DNA seqeunce of the plasmid used to transfrom the E. coli used to express the proteins was determined. The sequence data revealed that there was a mutation in the linker region between coding region for GST and the coding region for GFP, and that the mutation changed the amino acid code of the fusion protein.

What would you predict that you would seeon an SDS-PAGE gel if you ran a sample of the resin AFTER the elution step? Explain you answer. (4 marks)