You are about to start a cell biology experiment that requires a number of different chemical reagents. You have an unlimited supply of water and the following materials available in the lab to make your solutions; 500g dry glucose 1500 mL of 40% TRIS-buffer solution 150 mL of 0.3 molar ampicillin 2L of 35% SDS Using precise numerical values (to one decimal place, do NOT include units), describe how you will prepare 150 mL of 8% TRIS-buffer.

The answer is "212.5 ml"

Explanation:

Please find the complete question in the attached file.

In 2 ml of SDS it makes 120 ml of SDS.

To mix x ml of SDS

added water

So we have to add 24ml from SDS and add 96ml water to male 120ml SDS.

1500ml of 40% Tris Buffer solution  

Let us assume we have to mix y ml of 40% Tris buffer solution  

added water  

So we have to add 37.5 ml from 40% Tris Buffer and add 212.5 ml water to male 250 ml 6% Tris Buffer.

when—without warning—something provokes your anger, you may struggle not to succumb to it. since what typically makes you mad is feeling powerless in the face of what seems unfair, your anger is mostly an attempt at a “quick fix” to right the balance. it’s as though you’re raising a fist in protest, proclaiming that you’re not going to capitulate to such injustice.

there are, however, a multitude of problems related to this immediate, push-back reaction of anger. and probably the key one is that almost never does it resolve the issue that gave rise to it. such reactive anger is probably best understood as self-defeating. as david burns, the author of the seminal self- book feeling good, observes: if realistically, acting on your anger is to make any sense, it needs to meet two criteria—which, in almost every case, is frankly impossible. that is, your anger must:

be directed toward a person who has intentionally (and needlessly) behaved in a hurtful way toward you; and

be beneficial or advantageous to you (i.e., assist you in achieving a desired goal).

i think you’ll agree that only rarely can you claim that your anger is both warranted and , whether to yourself or the relationship.

so let me offer you a two-step alternative to abandoning your better judgment and giving in to the temptation of anger—one that should neutralize your anger in seconds. or, when you’re really angry, in minutes.

but keep in mind that you must really want to execute these steps, be sufficiently motivated to perform them. which means overcoming more unconscious resistance than you might ever have imagined. because there are many immediate “advantages” of anger that can interfere with your resolve, i’ll suggest a few of them that might interfere with your employing this powerful method to rid yourself of counter-productive anger.

explanation:

every chemical equation adheres to the law of conservation of mass, which states that matter cannot be created or destroyed. therefore, there must be the same number of atoms of each element on each side of a chemical equation.