QUESTION ONE (2 + 4 + 2 + 7 = 15)
1
t'AZ
2
3
toi
P
22
陳
W
2
=
i
a) In the chromatogram above what do X- and Y. axes represent?
b) Use the chromatogram above to differentiate the parameters tm, tr, t'r and w
c) The parameters k', a, N influence the separation of consecutive analyte bands, R.
Name each parameter "subject of the formulae in the Table below.
d) Make use of the chromatogram above and formulae in the Table below to calculate
ki, kz, a, N, and R. Assuming that each vertical interval on the X-axis is 1 minute,
make necessary approximations in your calculations
Name
Formula
Parameter