Chemistry, 15.02.2021 22:00 donuteatingcat

Consider an enzyme that uses general acid-base catalysis such that the reverse protonated form (call it HE) functions in one direction of the reaction, and the typically protonated form (call it EH) functions in the other direction of the reaction. Both the doubly protonated (call it HEH) and doubly unprotonated (call it E) forms of the enzyme are not effective in catalyzing either direction of the reaction. If the first macroscopic pKa of this enzyme (call it pK1') is 6.93, the second macroscopic pKa of this enzyme (call it pK2') is 9.13, and one of the microscopic pKas (pKA in the scheme below) is 7.50, then what proportion of the enzyme would be in the HEH form at pH 8.01

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Consider an enzyme that uses general acid-base catalysis such that the reverse protonated form (call...