Chemistry, 06.02.2021 04:30 Fionaauggies

You have a 5 kb vector and a 1 kb PCR fragment in separate solutions. You load 5 microliters of each into separate lanes on an agarose gel, and you compare the intensities by gel staining. The two bands appear to be of equal intensity. What volumes of vector and fragment would you add to a ligation reaction to have a 5:1 molar ratio of fragment to vector?

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You have a 5 kb vector and a 1 kb PCR fragment in separate solutions. You load 5 microliters of each...