A solution of transferrin was titrated with a standard solution of Fe3+. The blank absorbance was 0.04, and the absorbance of 10.00 mL of the transferrin solution measured after adding 6.00 mL of titrant was 0.65. Calculate the corrected absorbance that should be plotted.

The correct answer is 0.61.

Explanation:

Based on the given information, a transferrin solution was titrated with a standard ferric solution. The blank absorbance was 0.04 and the absorbance of 10 ml solution of transferrin post supplementation of 6 ml titrant came out to be 0.65. The corrected optical density or absorbance can be determined by using the formula,

Corrected OD = Measured OD - Blank or Reference OD

Now putting the values we get,

Corrected OD = 0.65 - 0.04

Corrected OD = 0.61