The purpose of this lab exercise will be to examine several factors involved in
photosynthesis.
-The effect of the intensity of light (# of photons per time) on the rate of
photosynthesis and know how to calculate the effect of light intensity on the rate
of photosynthesis
-Isolate and identify the photosynthetic pigments found in the chloroplasts of
spinach leaves - the separation of chloroplast pigments by paper chromatography.
Know how three factors affect capillary action: Molecular weight, Solubility, and
Affinity for the chromatography paper.
-Know what pigments travel the farthest up the paper chromatography:
Carotenes, Xanthophyll, chlorophyll-a, and Chlorophyll-b
-Calculate the Rf of the Paper Chromatography.
-Know how to operate the spectrophotometer.
-Construct an absorption spectrum of chloroplast extract from spinach leaves -
absorption spectrum of chloroplast extract and know what wavelengths are the
least and most strongly absorbed.
• The overall process of photosynthesis is summarized by:
• Notice photosynthesis uses energy (light energy) to make a more complex compound
(glucose). Therefore, photosynthesis is an anabolic and endergonic reaction.
• Cellular Respiration: The process by which the energy stored in food is converted to
ATP and heat. The overall process of cellular respiration is summarized by:
• Notice cellular respiration creates energy (ATP and heat) by breaking down a complex
compound (glucose). Therefore, cellular respiration is a catabolic and exergonic
reaction.
• Plants use special organelles called chloroplasts to absorb light. Chloroplasts contain
special pigments called photosynthetic pigments. A single plant may have different
photosynthetic pigments - each pigment absorbs a specific wavelength (color) of
light.
• In plants, what absorbs energy? Chlorophyll
• Chlorophyll is in chloroplast.
6 CO2 + 12 H2O + LIGHT C6H12O6 + 6 H2O + 6O2
• Photosynthesis
– Light energy from the sun is
captured and converted into
chemical energy (sugar)
– Occurs in chloroplasts in
mesophyll cells found in the
tissue of plant leaves
– Chlorophyll is the green
pigment of plants that
absorbs light energy
– CO2 enters and O2 exits
through small pores called
stomata
Photosynthesis
6 CO2 + 12 H2O + LIGHT C6H12O6 + 6 H2O + 6O2
• Photosynthesis: Is the process by which light energy is
converted into chemical energy. It occurs in all plants, algae,
and some bacteria. Photosynthesis provides oxygen to the
atmosphere.
• In this process, plants use light energy to convert carbon
dioxide and water into organic molecules and oxygen. In this
process, plants use light energy to convert carbon dioxide and
water into organic molecules and oxygen. The reactions of
photosynthesis occur in specialized structures called
chloroplasts.
• Light is radiated in very packets called photons, which travel
at specific wavelengths. We can distinguish among the
different wavelengths as differences in color. A prism is often
used to separate white light into its component colors.
Tracking atoms through photosynthesis
Photosynthesis splits water: using heavy oxygen (18)CO2 + 2 H2O CH2O + H2O + O2
CO2 + 2 H2O CH2O
Explanation:
that what i have
