(a) Given that the path length of the cuvette is 1 cm, what is the extinction coefficient of the 0.020 mM Yellow 5 dye at lambda max?(b) Given the data in the table below, what is the concentration of the diluted solution?Stock solution concentration 0.075 MStock solution absorbance 1.84Diluted solution absorbance 0.78

(a). Kindly check the explanation section.

(b). 24.5 M^-1 cm^-

(c). 0.0318 M

Explanation:

So, we are given the following data parameters which is going to aid us in solving the above Question;

The path length = 1cm, extinction coefficient = unknown, absorbance is unknown and concentration= 0.02mM.

Using the formula below; we can determine the extinction coefficient as;

Absorbance = extinction coefficient × concentration × path length. (1).

(a). Since, the absorbance is not given in the Question it won't be possible to determine the value of the extinction coefficient. Thus, say the value of absorbance = A.

Then, extinction coefficient = A/ 0.02 × 1 cm.

(b). Making use of the formula above, the extinction coefficient can be Determine as follows;

extinction coefficient = 1.84/0.075 × 1 = 24.5 M^-1 cm^-1.

(c). The concentration can be Determine by also making use of the formula above and making the concentration the subject of the formula;

Concentration = absorbance/ extinction coefficient × path length.

Concentration = 0.78/24.5 M^-1 cm^-1. × 1cm = 0.0318 M.

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