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Chemistry, 21.10.2020 19:01 jetblackcap

Mr. Knowlton loves to throw a football around for fun. In the summer, he and his brothers bought a new ball that was in perfect
condition. However, in the winter they noticed that the ball was not
the same. It seemed to have less volume even though there were no
leaks in the ball! Why?

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Answers: 3

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A3.37-mg sample of protein was chemically digested to convert its nitrogen into ammonia and then diluted to 100.0 ml. then 10.0 ml of this solution was placed in a 50-ml volumetric flask and treated with 5 ml of phenol solution plus 2 ml of sodium hypochlorite solution. the sample was diluted to 50.0 ml, and the absorbance at 625 nm was measured in a 1.00-cm cuvette and found to be 0.486. for reference, a standard solution was prepared from 10.0 mg of nh4cl (molar mass = 53.49 grams/mole) dissolved in 1.00 l of water. then 10.0 ml of this standard was placed in a 50-ml volumetric flask, treated in the same manner as the unknown, and the absorbance found to be 0.323. finally, a reagent blank was prepared using distilled water in place of unknown, it was treated in the same manner as the unknown, and the absorbance found to be 0.076. calculate the weight percent of nitrogen in the protein.
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Mr. Knowlton loves to throw a football around for fun. In the summer, he and his brothers bought a...

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