Determine which statements regarding aconitase are true. An equilibrium mixture contains less than 10% citrate, but the isocitrate dehydrogenase reaction is exergonic, and draws the aconitase reaction to the left. The iron of the iron-sulfur center forms a complex with the substrate that involves two hydroxyl groups from citrate. Aconitase is stereospecific. The reaction proceeds through the intermediate cis‑aconitate. Aconitase contains the iron‑sulfur center Fe2S2Fe2S2 .