DNA sequencing by the chain-termination method utilizes DNA polymerase I to make a complementary copy of the target or template DNA molecule. A reaction with a 20 bp template and dideoxyadenosine nucleotides as terminators results in the production of a 5 bp fragment. Based on this result, we can conclude that the template contains a thymine at position 16
b. a cytosine at position 16
c. a cytosine at position 5
d. a thymine at position 5
e. a uracil at position 5