Experiments performed with biological systems require a buffer that will not interfere with the stability and activities of the biological components. In 1966, Norman Good and his colleagues began a series of investigations that established criteria for new zwitterionic buffers that would be optimal for experimentation with biological systems. These criteria included:
a. pKa between 6 and 8
b. highly water soluble
c. minimally permeable to biological membranes
d. minimal effects of salt concentration
e. minimal effect of temperature on pKa (pKa/°C)
f. minimal effect of concentration on pKa minimal interaction with metal ions non-toxic
g. high chemical stabilit
h. minimal absorbance between 240-700 nm
For each buffer, choose the property that does not meet Good's criteria for biochemical buffers If all of the buffer's properties satisfy the criteria for a Good's buffer:
Properties of acetate buffer: Properties of MOPS buffer:
1. highly water soluble pKa at 25°C 7.15
2. ApKJ°C=-0.0002 ΔpKJ°C =-0.013
3. pKg at 25°C = 4.76 absorbs light at <230 nm
4. does not cross lipid bilayer does not cross lipid bilayer
5. all the above properties fulfill all the above properties fulfil
the lipid criteria the lipid criteria
Answers: 2
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Experiments performed with biological systems require a buffer that will not interfere with the stab...
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