For each set of dilutions (bleach and mouthwash), the first tube contained a 1:11 dilution (0.5 mL of agent was added to 5.0 mL of nutrient broth). What was the dilution of the MIC for the mouthwash and the bleach? Hint: You will have to calculate the dilution of each tube 2 – 4.

Tube 2: 8.26 * 10^-3; Tube 4: 6.83 * 10^-5

Explanation:

In the serial dilutions for MIC test, the volume of nutrient broth in each tube should be equal: 5.0 mL. And the volume of agent in each dilution should also be similar: 0.5 mL.

The serial dilutions was as following:

a rain shadow is a dry region of land on the side that is protected against the prevailing winds of a mountain range. the prevailing winds are the winds that occur most of the time in a particular place of the earth

prevailing winds carry air toward the mountain range. as the air rises up over a mountain range, the air cools, water vapor condenses, and clouds form. on this side of the mountains, called the windward side, precipitation falls in the form of  rain  or snow. this dry air produces a  rain shadow.