Tris(hydroxymethyl)aminomethane, commonly called or Trizma, is often used as a buffer in biochemical studies. Its buffering range is pH 7 to 9, and is for the aqueous reaction What is the optimal pH for buffers? pH = 8.1 Calculate the ratio at pH = 7.00 1 11.91 and at pH = 9.00. 83 10 (Simplify your answer completely.) A buffer is prepared by diluting 50.0 g base and 45.0 g hydrochloride (written as ) to a total volume of 2.0 L. What is the pH of this buffer? pH = What is the pH after 0.50 mL of 12 M is added to a 200.0-mL portion of the buffer?