Chemistry, 16.03.2020 23:59 mazielynn84

(20pt) (a)You are planning to produce a recombinant protein in E. coli when the cell density reaches 200 million cells/L, how long would you have to wait if you start with the initial inoculation density of 1,000 cells/L and a glucose concentration of 1g/L? (b) What is the final concentration of glucose? Given: for E. coli growth on glucose, µmax = 1.2 hr-1, Ks = 1x10-5 g/L, kd = 0.02 hr-1, cell mass of 1 cell = 1ng, YX/S = 0.6 g/g glucose

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(20pt) (a)You are planning to produce a recombinant protein in E. coli when the cell density reaches...