Chemistry, 10.03.2020 08:57 phancharamachasm

Which of the following rate-enhancing features does lysozyme use to increase the rate of the hydrolysis reaction it catalyzes?

Arrange the events for the hydrolysis of amide bonds by chymotrypsin in their correct order.

1. attack by Ser 196 to give a tetrahedral intermediate
2. enzyme returns to initial state
3. protonation by His 57 and then cleavage of the C-N bond release the C-terminal fragment
4. protonation by His 57 and then cleavage of the C-O bond release the N-terminal fragment
5. attack by water, leading to formation of a tetrahedral intermediate
6. binding of substrate to properly position the scissile bond for cleavage

a. 6,2,4, 3, 1, 5
b. 6, 1, 3, 5,4,2
c. 6, 1,3,5,2,4
d. 6, 1, 3,4, 5, 2

Part B:

Which of the following rate-enhancing features does lysozyme use to increase the rate of the hydrolysis reaction it catalyzes?
1. general acid-base catalysis
2. electrostatic catalysis
3. distortion of substrate
4. covalent catalysis

a. Only choices I, 2, and 4 are correct-
b. Only choices 1 and 2 are correct.
c. Only choices 1, 3, and 4 are correct.
d. Al of the listed choices are correct.

Answers: 3

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Which of the following rate-enhancing features does lysozyme use to increase the rate of the hydroly...