There are two main methods to get proteins into a gas phase for mass spectroscopy: electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Place the appropriate description of each ionization method in the appropriate space.- Can be used for proteins or peptides that have been treated with proteases like trypsin- Ionization evaporates solvent, leaving peptide in gas phase to travel through the separating mass spectrometer.- Uses mass-to-charge (m/z) ratio to determine molecular mass- Degrades and charges the peptide with high voltage- Proteins or peptides are mixed in a solid matrix rather than a solution.- Is based on the mass and acceleration though a chamber ending in a detector- Laser will be absorbed by the peptide containing material releasing the peptide into the gas phase.- Sample becomes ionized due to high voltage.- Peptides become fragmented and charged due to laser exposure.