1. (2pts) The breaking buffer that we use this week contains 10mM Tris, pH 8.0, 150mM NaCl. The elution buffer is breaking buffer that also contains 300mM imidazole. Describe how the instructor made the 0.25L elution buffer for all the students this week given 500ml of 1M of Tris (121.1 g/mole) (pH8.0), 750ml of 5M NaCl (MW = 58.44 g/mole) and 350ml of 1M imidazole (68.08 g/mole). Show your calculations for credit.

Which statement best explains the relationship between an area is geography and the temperature of its surface water

These questions are based on the attached photo. the experiment is about burning magnesium metal with oxygen. 1. write the balanced chemical equation for the reaction you are performing. 2. calculate the mass of magnesium metal used in each trial. o trial 1: o trial 2: 3. calculate the actual yield of magnesium oxide for each trial. o trial 1: o trial 2: 4. magnesium is the limiting reactant in this experiment. calculate the theoretical yield of mgo for each trial. o trial 1: o trial 2: 5. determine the percent yield of mgo for your experiment for each trial. o trial 1: o trial 2: 6. determine the average percent yield of mgo for the two trials. your company currently uses a process with a similar cost of materials that has an average percent yield of 91 percent. if the average percent yield of this process is higher than that, this could save the company money. what is your recommendation to the company? support your recommendation using your data, calculations, and understanding of stoichiometry gathered from this lab.

What is calcium oxide+diphosphorus pentoxide--> calcium phosphate balanced