Each ionizable group of an amino acid can exist in one of two states, charged or neutral. The electric charge on the functional group is determined by the relationship between its pKa and the pH of the solution. This relationship is described by the Henderson-Hasselbalch equation.

a) histidine has three ionizable functional groups with pKas of 2.0, 6.0, and 10.0 respectively. Draw the structure of histidine in each ionization state. What is the net charge on the histidine molecule in each ionization state?

b) using structures in (a) indicate the predominant ionization structures of histidine at pH 1, 4, 8, and 12. Note that the ionization state can be approximated by treating each ionizable group independently.

c) what is the net charge of histidine at pH 1, 4, 8, and 12? do not calculate fractional charges

d) briefly describe potential roles for the imidazole side chain of histidine in primary, secondary, tertiary, and quaternary protein structure.

Which of the following methods uses the decay of atomic particles in an object to find its exact age? a. fossil dating b. geologic dating c. radioactive dating d. relative dating

Straightforward questions answered in the powerpoint slidesreaction: heating the starting materials under refluxwhat does it mean to heat under reflux? why do we choose water as the reflux solvent? what are boiling chips used for? why do we put a condenser on top of the reaction? why do we add heat and let the reaction stir for 30 minutes? why do we add sulfuric acid to the reaction after it cools as opposed to when it’s still hot? separation: filtration of precipitatewhy don’t we do an aqueous and organic extraction in the separatory funnel? why do you rinse the salicylic acid on the filter with ice cold water? purification: recrystallization of salicylic acid (no hot filtration needed)what is the difference in the amount of room temperature water vs. boiling water needed to dissolve the salicylic acid (assume a 1.2 gram yield of salicylic acid)? remember, in the lab if you need x ml of boiling water to dissolve a solid, then you should add a little more (definitely no more than 1.5 times the theoretical amount) to ensure it doesn’t recrystallize prematurely. analysis: melting point of salicylic acidwhat can you conclude if the melting point of the salicylic acid you just synthesized is 152-155oc and the 1: 1 mix of your product and “synthetic” salicylic acid is 151-154oc?

Nebulae are enormous clouds in outer space. they are made mostly of hydrogen gas, helium gas, and dust. some nebulae glow brightly, while others do not. the stars that people see are huge, bright balls of glowing gas. they are made mostly of hydrogen and helium. which statement correctly describes other ways in which nebulae and stars are different? a. stars can form inside a nebula but a nebula can never be produced by any star. b. a star always has a higher density than a nebula. c. stars can never form inside a nebula but a nebula can be produced by any star. d. a nebula always has a higher density than a star.