You have 100ml of 1M Glycine (total Glycine) and you want to make a buffer at pH = 3.2. Assuming normal pKa values for amino acids, which functional group would be relevant for this buffer, and how much Glycine in the unprotonated versus protonated state would you need? (HINT: what is the ratio of unprotonated versus protonated at pH = 3.2?)
a. Amine, 9mM B / 91mM BH+b. Amine, 91 mM B / 9mM BH+c. Acid, 9mM A- / 91mM HAd. Acid, 91mM A- / 9mM HA