Use henderson-hassalbach equation. the pka of tris-hcl is 8.1. after you have added 1n hcl to adjust the ph of the tris solution to 8.1 what is the ratio (in mmols) of tris to tris-hcl in the resulting buffer solution?

Explanation:

According to the Henderson-Hassalbach equation for pH is as follows.

        pH =

It is given that the pH and are same.

Hence,    

        = 0

      =

                                 = 1

Hence, whenever the pH and are same then the ratio of conjugate base to acid is 1.

Thus, we can conclude that the ratio of Tris to Tris-HCl is 1.