To determine the isoelectric point of a protein, first establish that a gel: a) contains a denaturing detergent that can distribute uniform negative charges over the protein surface. b) exhibits a stable ph gradient when ampholytes become distributed in an electric field. c) is washed with an antibody specific to the protein of interest. d) neutralizes all ionic groups on a protein by titrating them with strong bases. e) relates the unknown protein to a series of protein markers with known molecular weights.

The correct answer is B.

Explanation:

To determine the isoelectric point of a protein, first establish that a gel exhibits a stable pH gradient when ampholytes are distributed in an electric field. The isoelectric point is the pH at which a polyampholyte has a net charge of zero. At this pH value, the solubility of the substance is almost zero. Thus, the gel must show a stable pH gradient.

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it can be observed without changing the identity of the object.

it depends on what substance you're talking about as well as many other factors. in quantum mechanics, the observation of particles affects the observers ability to measure certain properties. for example: you can know the velocity of a particle, but you can't know it's position; or you can know it's position, but not it's velocity. it's like trying to find out where a grain of sand is by throwing other grains of sand at it. once you find out where it is, it's been moved. it's also like trying to find an answer to the question of whether or not a tree falling in a forest makes a sound if nobody is there to hear it. paradoxically, the only way to find out is to be there and hear it.