What would be the order of elution (from first --> last) of the proteins carbonic anhydrase (pi = 7.0), carboxypeptidase b (pi = 6.2) and chymotrypsin (pi = 8.0) run on a cm (carboxymethyl) ion exchange column at ph 6.6 eluted with an increasing nacl gradient:
carboxypeptidase b, carbonic anhydrase, chymotrypsin.
chymotrypsin, carboxypeptidase b, carbonic anhydrase.
carboxypeptidase b and carbonic anhydrase will elute together followed by chymotrypsin.
carbonic anhydrase, chymotrypsin, carboxypeptidase b.
chymotrypsin, carbonic anhydrase, carboxypeptidase b.
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