Critical thinking questions 1. which technique(s) could you utilize to separate two proteins that (a) differ greatly by size, (b) differ by pl, and (c) that have similar physical characteristics (e. g., size and pi), but have very different functional characteristics? in each case briefly describe how the separation would be accomplished. (a)

answer : one can design an experiment using a liquid and an additive that can be added to the liquid and observe the chemical changes in the reaction species.

one would need a beaker which is filled with a known amount of the liquid in which thermometer is placed to measure the temperature changes; after addition of the additive to the liquid solution one can note down the temperature change and find whether the reaction is endothermic or exothermic in nature. by subtracting the initial temperature value with the final temperature one can find the temperature changes.

various combinations of liquid and additive can be used. liquid in this case can be a known quantity of water and additives can be epsom salt or without epsom salt the experiment can be conducted to notice the changes. water and ice, hydrogen peroxide and dry yeast, vinegar and baking soda,etc.

answersilicon (si); oxygen (o);

the answer is a! (2.9 x 10^-18 j) hope i could someone. : )