What is the simple and accurate way, other than density, to distinguish the organic phase from the aqueous phase in the experiment that you have performed?

Addition of the little amount of the water

Explanation:

Other than density, the another method which can be used to distinguish between the organic phase from the aqueous phase is:

A little amount of the water is added to the separatory funnel and notice where the water droplets are going.

If top layer is the aqueous layer, water droplets mix with top layer, and disappear. If bottom layer is the aqueous layer, water droplets will pass through top layer so to mix with bottom layer. This motion can help to distinguish.

However, if it is difficult to track where water droplets go, keep the track of volume of layers: whichever the layer increases with addition of the water is aqueous layer.

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