Chemistry, 24.06.2019 10:40 firenation18
The following data on baker’s yeast in a particular medium at 23.4°c were obtained in the presence and in the absence of an inhibitor, sulfanilamide. the reaction rate (-rs) was measured in terms of the oxygen uptake rate qo2, obtained as a function of oxygen partial pressure. (a) assume the rate qo2 follows michaelis-menten kinetics with respect to oxygen. calculate the qo2 maximum (i. e. vmax), and the michaelis-menten constant km. (b) using the lineweaver-burk plot, determine the type of inhibition sulfanilamide that causes the o2 uptake to change. po2 * qo2 (no sulfanilamide) qo2 (20 mg sulfanilamide/ml added to medium) 0.0 0.0 0.0 0.5 23.5 17.4 1.0 33.0 25.6 1.5 37.5 30.8 2.5 42.0 36.4 3.5 43.0 39.6 5.0 43.0 40.0 *po2 = oxygen partial pressure, mmhg; qo2 = oxy
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The following data on baker’s yeast in a particular medium at 23.4°c were obtained in the presence a...
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