When you added primary antibody to the wells, what happened if your sample contained the antigen? what if it did not contain the antigen? when you added secondary antibody to the wells, what happened if your sample contained the antigen? what if it did not contain the antigen? if the sample gave a negative result for the antigen, does this mean that the antigen is not present? what reasons could there be for a negative result when the antigen is actually present?

When I added a primary antibody to a well with a sample which contained the antigen, it bind to the antigen but could not bind with the antigen if it was not present for it would be flashed out in the wash step.

Adding secondary antibody, it produced a color change to the antigen because the enzyme bound secondary antibody, would bind to the primary antibody already bound to antigen in the wells. If the antigen was not present, there would be no binding in the wells thus the secondary antibody would have nothing to bid to and would be flashed out in the wash step.

answer:

sequence 1- deletion

                  2- duplication

                  3- insertion

                  4- substitute

                  5- inversion