You decide to clone a gene using a plasmid vector featuring ampicillin resistance and lacz. however, when you try to grow the e. coli onto a plate containing x-gal and ampicillin, you see no colonies. explain what most likely went wrong during the cloning process.

Ectocarpene is a volatile, sperm cell-attracting material released by the eggs of the seaweed ectocarpus siliculosus. its constitution is all the double bonds are cis, and the absolute configuration of the chirality center is s. draw a stereochemically accurate representation of ectocarpene.

How many major plates cover the earth

He did not infringe upon the policy making powers that he felt the constitution gave congress. but the determination of foreign policy became preponderantly a presidential concern. when the french revolution led to a major war between france and england, washington refused to accept entirely the recommendations of either his secretary of state thomas jefferson, who was pro-french, or his secretary of the treasury alexander hamilton, who was pro-british. rather, he insisted upon a neutral course until the united states could grow stronger. which provides the best objective summary of this excerpt? president washington made a good choice when he decided not to accept the recommendations of his advisors and instead insisted that the united states stay neutral during the war between france and england as president, washington believed that the united states should remain neutral in foreign policy even though he had advisors who recommended that he take sides during the war between france and england. washington did not make policy decisions during his presidency because he did not want to interfere with the policy-making powers that the constitution gave congress. washington refused to accept entirely the recommendations of either his secretary of state thomas jefferson, who was pro-french, or his secretary of the treasury alexander hamilton, who was pro-britis