Enzymes can be purified from the Golgi apparatus. The gene for the enzyme can be cloned and fused to the coding sequence of GFP to produce a GFP fusion protein. The GFP fusion protein can then be expressed in cell culture. Antibodies to GFP are readily available. To determine which region of the Golgi apparatus, cis, medial or trans, the protein is located in, the protein can be visualized by
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Enzymes can be purified from the Golgi apparatus. The gene for the enzyme can be cloned and fused to...
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