3. Ligation of the 784-bp fragment, 3755-bp fragment, 1875-bp fragment and 2332-bp fragment produces a "double plasmid" pAMP/pKAN. Make a scale drawing of the double plasmid pAMP/pKAN. 4. What kinds of antibiotic selection would identify E. coli cells that have been transformed with each of the

plasmids drawn in Questions 1-3?

1. environmental factors, such as ph and temperature, can affect the function of an enzyme. a. true b. false 2. which of the following is an organic molecules (ex. vitamins) that an enzyme? a. coenzyme b. product c. active site d. substrate 3. how do enzymes effect the speed of a chemical reaction? a. they speed it up b. they slow it down c. they have no effect on the speed of a reaction 4. during a chemical reaction, an enzyme is used up so that it can only be used one time. a. true b. false 5. which of the following results from a chemical reaction that is catalyzed by an enzymes? a. active site b. product c. substrate d. coenzyme

Seen in some gram-positive bacilli, occurs when only the inner portion of a cell wall is deposited across the dividing cell. this new cross wall puts tension on the outer layer of the old cell wall. eventually, the outer wall breaks at its weakest point with a snapping movement that tears it most of the way around. the daughter cells can then remain hanging together almost side by side being held by a small remnant of the original outer wall. choose from the following statements the ones that correctly discuss reproduction using binary fission in a bacterial cell. select all that apply. view available hint(s) select all that apply. due to the stretching of the cytoplasmic membrane, both cells will contain a complete genome. each daughter cell will contain an equal number of organelles. the daughter cell will be a permanently smaller copy of the mother cell but will contain a complete genome. each daughter cell is an exact copy of the other, both genetically and morphologically

Which part of cellular respiration must occur before any of the other steps can occur