You are planning to split a T25 flask of CHO cells that contained 5 ml of media. You removed all of the old media and washed the cells with PBS. The PBS was then removed and 1 ml of trypsin was added to disloge the cells. After 10 minutes, 4 ml of fresh media was added to the flask. 100 ul of cells were removed from the flask and added to 700 ul of fresh media in a microfuge tube. The tube was mixed and then 100 ul of cell suspension was removed and added to 100 ul of trypan blue. Using the countess you received a count of 4.5 x 10^4 cells/ml. What is actual concentration of cells in the T25 flask, after the trypsin and fresh media was added

9.9 years

a = p(1 + rt) . . account balance after time t at rate r starting with principal p

3000 = 2500(1 + 0.0202t) . . filling in the given numbers

1.2 = 1 + 0.0202t . . divide by 2500

0.2 = 0.0202t . . . subtract 1

0.2/0.0202 = t ≈ 9.901

it will take about 9.9 years for the account balance to reach $3000.

the correct answer is b

the scientific method.

when you breathe in, or inhale, your diaphragm contracts (tightens) and moves downward. this increases the space in your chest cavity, into which your lungs expand. the intercostal muscles between your ribs also enlarge the chest cavity. they contract to pull your rib cage both upward and outward when you inhale.