What are the features of CRISPRa Stable Cell Lines? CRISPRa (CRISPR activation) system is a technology based on the improved CRISPR/Cas9 system that uses a synthetic programmable transcription factor to initiate endogenous transactivation of genes. In mammalian cells, the expressing of dCas9 (a nuclease-deficient Cas9 only retaining DNA binding activity) fused with a transcriptional activation domain, and gRNA complementary to the target promoter region in the genome, will result in increased transcription of the corresponding gene.

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Considering the yellow and green pea color phenotypes studied by gregor mendel: a. what is the biochemical function of the protein that is specified by the gene responsible for the pea color phenotype? (1 point) b. a null allele of a gene is an allele that does not specify (or encode) any of the biochemical function that the gene normally provides (in other words, either no protein at all or only non-functional protein is produced from it). of the two alleles, y and y, which is more likely to be a null allele? (1 point) c. in terms of the underlying biochemistry, why is the y allele dominant to the y allele? (2 points) d. why are peas that are yy homozygotes green? (1 point) e. the amount of protein produced from a gene is roughly proportional to the number of functional copies of the gene carried by a cell or individual. what do the phenotypes of yy homozygotes, yy heterozygotes, and yy homozygotes tell us about the amount of sgr enzyme needed to produce a yellow color? explain your reasoning. (2 points)

Energy stored in the bonds that hold together the atoms and molecules of all substances is called a. kinetic energy. b. electrical energy. c. mechanical energy. d. chemical energy.

Light sensitivity in euglena is provided by the