Below is the sequence of the HEXA gene with the underlined portion the 1278TATC allele that recessively caused Tay-Sachs disease. Answer the following questions about this sequence: 5’ 3’

3’ 5’

a. Identify all the PAM sites by clearly marking them in the sequence above.
b. Pick one that is appropriate for the goal of removing the 4 extra TATC nucleotides. There are multiple good answers and you should only choose one.
c. Design a 20bp sgRNA spacer sequence that will make an appropriate cut. There are multiple good answers, and you should only choose one. Be sure to label 5’ and 3’ and the side that would attach to the scaffold sequence.
d. Would you use an HDR template or NHEJ to consistently make the correct edit? Explain

1: the largest region of the brain is the:

  cerebrum

2. the term "psychology" literally means:

the process or procedure of the science or study of the mind.

3. the area of the brain responsible for respiration, heart rate, and smooth muscle control of our blood vessels is t

medulla oblongata

4. the structure that conducts nerve impulses to and from the brain and serves as a reflex center for some sensory information without input from the brain is the:

spinal cord

5. the three-layered membrane that covers the brain and spinal cord and literally means membrane is called the:

meninges

6. the cord-like (string-like) structures that carry bio-electrical impulses from one part of the body to another are called

nerves

7. a group of nerve cell bodies that are found outside of the spinal cord and brain are referred to as:

ganglions or ganglia (pl.) “literal meaning knot”

8. the term that means spinal cord inflammation is:

myel/itis

the answer that best describes the relative refractory period in the neuron is the resting membrane potential is restored, but concentrations of sodium and potassium are not restored to their original state.

the refractory period is the time during which another stimulus given to the neuron will not lead to a second potential. thus, because na+ channels are inactivated during this time, additional depolarizing stimuli do not lead to new action potentials.  

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b is the correct answer

true is the