Biology, 08.08.2021 08:40 angela563

Describe 2 limitations of a 3D cell model.

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Traditional Pathology: Traditional pathology involves removing cell culture models from well plates and processing them. Despite the fact that this method may obtain pathological information from tissues, the scale of these models makes the process extremely low-throughput, time-consuming, and costly.

Dissolution Based Assays: Many researchers are currently using dissolution based assays where cell culture models are homogenized and analyzed using a fluorescence probe such as Promega’s CellTiter-Glo Luminescent kit. While these assays are high-throughput, they do not capture the 3D data held within these models which makes them intrinsically more valuable than traditional 2D cell culture models.

Answer from: veroushkarose7326

3D structures is the fact that “spheres” can be formed, not from a single cell, but from a few cell clusters. However, even structures created as aggregates of several cells still have a three-dimensional form and seem to be a better model than flat, adherent cultures

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Answer from: jg333

Until recently, the investigation of the molecular mechanisms, involved in the response of tumor cells to various anti-tumor agents, were performed on cells grown on two-dimensional (2D) surfaces. The cells cultured on a 2D flat-surface, usually made of polystyrene, do not grow similar to natural conditions, and this affects the cell-to-cell interactions, the cell behavior, and response to different external stimuli [2][3][4]. Thus, developing the new cell culture models to better reflect the chemosensitivity of carcinoma cells to anti-tumor drugs is in high demand. The three-dimensional (3D) culture systems were envisaged to mimic the in vivo situation and create a close to the native environment in which the cells grow and interact with their neighbors and with the surrounding matrix components in all three dimensions like the cells do in the tissue microenvironment.

Answer from: Quest

Can you write it in english

Answer from: Quest

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