The researcher places the lacZ gene in the cloning region so that it is disrupted by DNA inserts. Place the remaining steps for this process in the correct order. Not all steps will be used. a. Blue colonies that survive On the culture medium contain the recombinant plasmid.
b. Grow cells containing the recombinant plasmid in a medium with ampicillin and X-gal (a galactose derivative).
c. All the fragments have the sticky ends 5 '—GCGGCCGC—3'.
d. All the fragments have identical sticky ends: 5' —GGCC—3'.
e. Use DNA ligase to join the sticky ends of the DNA sample and the plasmid.
f. White colonies that survive on the culture medium contain the recombinant plasmid.
g. Remove colonies containing the recombinant plasmid from the culture medium and continue growing.