You wish to develop a monoclonal antibody (mAb) that binds with high affinity to the SARS-CoV-2 spike (S) protein, for use as a therapeutic agent. You have isolated a human B-cell clone that produces an IgG that is specific for the SARS-CoV-2 S protein, but its affinity for binding the target epitope is low. Describe in conceptual detail how you could use a protein engineering approach to enhance the affinity of the mAb for the target antigen.​

Atp is is our little "pocket" of energy. of course, if you have too little, then it means you have too little energy, and you'll pass out. the way to not have enough, is to have no energy sources (glucose, fats, proteins). you can't have too many atp molecules. cycles like the krebs cycle produce it, but if you have too much of any substrate (or too little for that matter), the cycle stops and atp is no longer made (it stops until substrate levels are such that the process can go on). in any case, this is unlikely since atp is constantly hydrolyzed and remade, even while you're asleep. you won't have too much. if you have zero, you are dead or dying.